AB0061 ALTERATIONS OF XANTHINE OXIDOREDUCTASE ACTIVITY IN RED BLOOD CELLS AFTER GLUCOCORTICOID TREATMENT IN RHEUMATOID ARTHRITIS

Background: According to modern concepts, rheumatoid arthritis (RA) refers severe autoimmune rheumatic diseases. The activation of free radical oxidation processes is essential in the development this disease [1]. Xanthine oxidoreductase a significant reactive oxygen species source [2]. Despite great advances treatment associated with introduction innovative drugs and especially improvement strategy for their use into clinical practice, glucocorticoids still remain an important component RA pharmacotherapy actual practice. Objectives: evaluate changes activities xanthine interconvertible forms (xanthine oxidase, ЕС 1.17.3.2 dehydrogenase, 1.17.1.4) lysed red blood cells patients relation glucocorticoid treatment. Methods: 47 verified 30 healthy controls were enrolled study. diagnosis was using 2010 ACR/EULAR criteria 2010. All have moderate DAS28 scores. randomized 2 groups comparable gender, age principal manifestations. Methylprednisolone (Metipred, Orion Corp.), average dose mg/day, betamethasone (Diprospan, Schering-Plough), single dose7 mg, administered intramuscularly respective groups. Хanthine oxidase (XO) dehydrogenase (XDG) measured by spectrophotometric method as previously described [3]. these enzymes studied before after injection glucocorticoids. Statistical comparison tests selected according common guidelines, differences considered when p<0.05. Central tendencies expressed means±SEM. Results: Mean methylprednisolone group 41.8±1.05 years, mean duration (± SEM) 7.9±0.21 years. diprospan 40.9±1.07 8.0±0.33 Significant decreases XO activity increase XDG observed just each drug. Changes enzymatic more pronounced group. However did not reach level controls. As previously, decreased increased plasma therapeutic doses glucocorticoids, well lymphocytes [4]. Conclusion: Treatment can affect balance XO/XDG antioxidant potential blood. This effect exert beneficial influence on inflammation RA. References: [1]Mateen S., et al. Increased formation oxidative stress arthritis. PLoS ONE 2016;11(4):e0152925. [2]Çimen M.Y., Oxidant/antioxidant status erythrocytes from Clin Rheumatol 2000;19(4):275-277. [3]Zborovskaya I.A., Influence analgetics lymphocytic purine metabolism patients. Russian Journal Pain 2018;3:47. [4]Mozgovaya E.E., Xanthinoxidase Osteoporosis International 2019;30(2):S433-434. Disclosure Interests: None declared